Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription
نویسندگان
چکیده
منابع مشابه
8-Hydroxy-2-methylquinoline
The asymmetric unit of the title compound, C(10)H(9)NO, contains two independent mol-ecules which are linked by a pair of O-H⋯N hydrogen bonds into a hydrogen-bonded dimer.
متن کاملEffects of UV Radiation and Oxidative DNA Adduct 8-hydroxy-2’-deoxiguanosine on the Skin Diseases
Excessive sun exposure may be associated with increased oxidative stress and oxidative damage of DNA. Based on this hypothesis, the authors focused on investigating the causal role of UV-induced genomic damage in the pathophysiology of porphyria cutaneatarda (photosensitive acquired metabolic disorder with autosomal dominanttransmittion). In this study we demonstrated that serum levels of 8-hyd...
متن کاملMutagenic effects of 8-hydroxy-dGTP in live mammalian cells.
The mutagenicity of an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP), was examined using COS-7 cells. 8-OH-dGTP and supF shuttle plasmid DNA were cointroduced by means of cationic liposomes, and the DNAs replicated in the cells were recovered and then transfected into Escherichia coli. 8-OH-dGTP induced A:T-->C:G substitution mutations in the COS-7 cells. This r...
متن کاملHuman MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP.
The human nucleotide pool sanitization enzyme, MTH1, hydrolyzes 2-hydroxy-dATP and 8-hydroxy-dATP in addition to 8-hydroxy-dGTP. We report here that human MTH1 is highly specific for 2-hydroxy-ATP, among the cognate ribonucleoside triphosphates. The pyrophosphatase activities for 8-hydroxy-GTP, 2-hydroxy-ATP and 8-hydroxy-ATP were measured by high-performance liquid chromatography. The kinetic ...
متن کاملUrinary Sulfate, Asymmetric Dimethylarginine, and 8-Hydroxy-2'-Deoxyguanosine:
Compounds found in the urine represent the end products of metabolism and are the expression of the efficiencies or inefficiencies of this process. Nutritional status has a direct effect on this metabolic efficiency. Careful assessment of certain urinary compounds can yield valuable information on the nutrient needs of the individual. Urinary sulfate, asymmetric dimethylarginine (ADMA), and 8-h...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Free Radical Biology and Medicine
سال: 2007
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2007.05.034